沃辛顿新美国生理学会（APS）技术网络研讨会

在本次网络研讨会上，德克萨斯大学奥斯汀分校的Roopa Ravichandar博士和Sam Faraguna将分享他们对分离和培养神经元细胞方案的研究。  

他们将讨论各种方法如何支持神经系统发育过程中神经元兴奋性的研究。

节目亮点将包括背根神经节神经元的分离和免疫扫描方案，以及视网膜神经节细胞如何成为研究眼睛发育过程中调节神经元电特性的因素的强大模型的演示。本次会议还将提供关于使用免疫扫描获得高纯度神经元培养物的有效分离技术的实用指导。

主要学习目标：

分离大鼠背根神经节细胞的方法。
从大鼠视网膜组织中分离纯视网膜神经节细胞的优化。
使用这些神经元细胞类型研究神经元兴奋性的功能应用。

In this webinar, Roopa Ravichandar, PhD, and Sam Faraguna of the University of Texas at Austin will share their research on protocols for isolating and culturing neuronal cells.  

They will discuss how various methods support studies of neuronal excitability during nervous system development.

Program highlights will include dissociation and immunopanning protocols of dorsal root ganglion neurons and a demonstration of how retinal ganglion cells are a powerful model for investigating factors that regulate neuronal electrical properties during eye development. The session will also feature practical guidance on effective isolation techniques using immunopanning to obtain highly purified neuronal cultures.

Key Learning Objectives:

• Methods to isolate rat-derived dorsal root ganglion cells.
• Optimization of isolating pure retinal ganglion cells from rat-derived retinal tissue.
• Functional applications of using these neuronal cell types to study neuronal excitability.