脱氧核糖核酸酶I

脱氧核糖核酸酶I（DNase I）是一种内切核酸酶，催化DNA中磷酸二酯键的水解，生成带有5'-磷酸和3'-羟基末端的寡核苷酸。它需要二价阳离子（Mg2+或Mn2+）维持活性。牛胰腺来源的该酶广泛应用于分子生物学研究，包括RNA分离、细胞培养和染色质免疫沉淀（ChIP）实验。

Deoxyribonuclease I (DNase I) is an endonuclease that catalyzes the hydrolysis of phosphodiester linkages in DNA, producing oligonucleotides with 5'-phosphate and 3'-hydroxyl termini. It requires divalent cations (Mg2+ or Mn2+) for activity. The bovine pancreatic enzyme is widely used in molecular biology research, including RNA isolation, cell culture, and chromatin immunoprecipitation (ChIP) assays.

EC Number: 3.1.21.1